5BUD

Crystal structure of Candida albicans Rai1 in complex with pU5-Mn2+

5BUD の概要

• エントリーDOI: 10.2210/pdb5bud/pdb
• 分子名称: Decapping nuclease RAI1, RNA (5'-R(P*UP*UP*UP*U)-3'), MANGANESE (II) ION, ... (4 entities in total)
• 機能のキーワード: rai1, rna, decapping, mrna 5'-processing, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 95069.81

構造登録者

Wang, V.Y.,Tong, L. (登録日: 2015-06-03, 公開日: 2015-07-08, 最終更新日: 2024-03-06)

主引用文献

Wang, V.Y.,Jiao, X.,Kiledjian, M.,Tong, L.
Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes.
Nucleic Acids Res., 43:6596-6606, 2015

PubMed Abstract: Recent studies showed that Rai1 and its homologs are a crucial component of the mRNA 5'-end capping quality control mechanism. They can possess RNA 5'-end pyrophosphohydrolase (PPH), decapping, and 5'-3' exonuclease (toward 5' monophosphate RNA) activities, which help to degrade mRNAs with incomplete 5'-end capping. A single active site in the enzyme supports these apparently distinct activities. However, each Rai1 protein studied so far has a unique set of activities, and the molecular basis for these differences are not known. Here, we have characterized the highly diverse activity profiles of Rai1 homologs from a collection of fungal organisms and identified a new activity for these enzymes, 5'-end triphosphonucleotide hydrolase (TPH) instead of PPH activity. Crystal structures of two of these enzymes bound to RNA oligonucleotides reveal differences in the RNA binding modes. Structure-based mutations of these enzymes, changing residues that contact the RNA but are poorly conserved, have substantial effects on their activity, providing a framework to begin to understand the molecular basis for the different activity profiles.

PubMed: 26101253
DOI: 10.1093/nar/gkv620

実験手法

X-RAY DIFFRACTION (1.99 Å)