5BSZ

X-ray structure of the sugar N-methyltransferase KedS8 from Streptoalloteichus sp ATCC 53650

5BSZ の概要

• エントリーDOI: 10.2210/pdb5bsz/pdb
• 分子名称: N-methyltransferase, S-ADENOSYL-L-HOMOCYSTEINE, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: n-methyltransferase kedarcidin trideoxysugar s-adenosylmethionine, transferase
• 由来する生物種: Streptoalloteichus sp. ATCC 53650
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27989.47

構造登録者

Thoden, J.B.,Delvaux, N.A.,Holden, H.M. (登録日: 2015-06-02, 公開日: 2015-07-29, 最終更新日: 2023-09-27)

主引用文献

Delvaux, N.A.,Thoden, J.B.,Holden, H.M.
Molecular architecture of KedS8, a sugar N-methyltransferase from Streptoalloteichus sp. ATCC 53650.
Protein Sci., 24:1593-1599, 2015

PubMed Abstract: Kedarcidin, produced by Streptoalloteichus sp. ATCC 53650, is a fascinating chromoprotein of 114 amino acid residues that displays both antibiotic and anticancer activity. The chromophore responsible for its chemotherapeutic activity is an ansa-bridged enediyne with two attached sugars, l-mycarose, and l-kedarosamine. The biosynthesis of l-kedarosamine, a highly unusual trideoxysugar, is beginning to be revealed through bioinformatics approaches. One of the enzymes putatively involved in the production of this carbohydrate is referred to as KedS8. It has been proposed that KedS8 is an N-methyltransferase that utilizes S-adenosylmethionine as the methyl donor and a dTDP-linked C-4' amino sugar as the substrate. Here we describe the three-dimensional architecture of KedS8 in complex with S-adenosylhomocysteine. The structure was solved to 2.0 Å resolution and refined to an overall R-factor of 17.1%. Unlike that observed for other sugar N-methyltransferases, KedS8 adopts a novel tetrameric quaternary structure due to the swapping of β-strands at the N-termini of its subunits. The structure presented here represents the first example of an N-methyltransferase that functions on C-4' rather than C-3' amino sugars.

PubMed: 26177844
DOI: 10.1002/pro.2742

実験手法

X-RAY DIFFRACTION (2 Å)