5BQX

Crystal structure of human STING in complex with 3'2'-cGAMP

5BQX の概要

• エントリーDOI: 10.2210/pdb5bqx/pdb
• 関連するPDBエントリー: 4KSY
• 分子名称: Stimulator of interferon genes protein, 3'2'-cGAMP (3 entities in total)
• 機能のキーワード: sting, 3'2'-cgamp, immune system
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27855.06

構造登録者

Wu, J.,Zhang, X.,Chen, Z.J.,Chen, C. (登録日: 2015-05-29, 公開日: 2015-06-24, 最終更新日: 2024-03-06)

主引用文献

Shi, H.,Wu, J.,Chen, Z.J.,Chen, C.
Molecular basis for the specific recognition of the metazoan cyclic GMP-AMP by the innate immune adaptor protein STING.
Proc.Natl.Acad.Sci.USA, 112:8947-8952, 2015

PubMed Abstract: Cyclic GMP-AMP containing a unique combination of mixed phosphodiester linkages (2'3'-cGAMP) is an endogenous second messenger molecule that activates the type-I IFN pathway upon binding to the homodimer of the adaptor protein STING on the surface of endoplasmic reticulum membrane. However, the preferential binding of the asymmetric ligand 2'3'-cGAMP to the symmetric dimer of STING represents a physicochemical enigma. Here we show that 2'3'-cGAMP, but not its linkage isomers, adopts an organized free-ligand conformation that resembles the STING-bound conformation and pays low entropy and enthalpy costs in converting into the active conformation. Our results demonstrate that analyses of free-ligand conformations can be as important as analyses of protein conformations in understanding protein-ligand interactions.

PubMed: 26150511
DOI: 10.1073/pnas.1507317112

実験手法

X-RAY DIFFRACTION (2 Å)