5BQL

Fluorescent protein cyOFP

Summary for 5BQL

• Entry DOI: 10.2210/pdb5bql/pdb
• Descriptor: Fluorescent protein cyOFP (2 entities in total)
• Functional Keywords: fluorescent protein
• Biological source: Entacmaea quadricolor
• Total number of polymer chains: 4
• Total formula weight: 110001.64

Authors

Sens, A.,Ataie, N.,Ng, H.L.,Lin, M.Z.,Chu, J. (deposition date: 2015-05-29, release date: 2016-06-01, Last modification date: 2019-12-18)

Primary citation

Bajar, B.T.,Wang, E.S.,Zhang, S.,Lin, M.Z.,Chu, J.
A Guide to Fluorescent Protein FRET Pairs.
Sensors Basel Sensors, 16:-, 2016

PubMed Abstract: Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies.

PubMed: 27649177
DOI: 10.3390/s16091488

Experimental method

X-RAY DIFFRACTION (2.39 Å)