5BQL

Fluorescent protein cyOFP

5BQL の概要

• エントリーDOI: 10.2210/pdb5bql/pdb
• 分子名称: Fluorescent protein cyOFP (2 entities in total)
• 機能のキーワード: fluorescent protein
• 由来する生物種: Entacmaea quadricolor
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 110001.64

構造登録者

Sens, A.,Ataie, N.,Ng, H.L.,Lin, M.Z.,Chu, J. (登録日: 2015-05-29, 公開日: 2016-06-01, 最終更新日: 2025-04-02)

主引用文献

Bajar, B.T.,Wang, E.S.,Zhang, S.,Lin, M.Z.,Chu, J.
A Guide to Fluorescent Protein FRET Pairs.
Sensors Basel Sensors, 16:-, 2016

PubMed Abstract: Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor and acceptor fluorophores in FRET biosensors, especially since FPs are genetically encodable and live-cell compatible. In this review, we will provide an overview of methods to measure FRET changes in biological contexts, discuss the palette of FP FRET pairs developed and their relative strengths and weaknesses, and note important factors to consider when using FPs for FRET studies.

PubMed: 27649177
DOI: 10.3390/s16091488

実験手法

X-RAY DIFFRACTION (2.39 Å)