5BOA

Crystal Structure of the Meningitis Pathogen Streptococcus suis adhesion Fhb bound to the disaccharide receptor Gb2

5BOA の概要

• エントリーDOI: 10.2210/pdb5boa/pdb
• 関連するPDBエントリー: 5BOB
• 分子名称: Translation initiation factor 2 (IF-2 GTPase), alpha-D-galactopyranose-(1-4)-beta-D-galactopyranose (2 entities in total)
• 機能のキーワード: protein-polysaccharide complex, sugar binding protein
• 由来する生物種: Streptococcus suis (strain 05ZYH33)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 155558.90

構造登録者

Zhang, C.,Yu, Y.,Yang, M.,Jiang, Y. (登録日: 2015-05-27, 公開日: 2016-05-04, 最終更新日: 2023-11-08)

主引用文献

Zhang, C.,Hao, H.,Yu, Y.,Kong, D.,Chen, S.,Jiang, H.,Yuan, Y.,Zheng, Y.,Yang, M.,Jiang, Y.
Structural basis of the interaction between the meningitis pathogen Streptococcus suis adhesin Fhb and its human receptor.
Febs Lett., 590:1384-1392, 2016

PubMed Abstract: The recently identified Streptococcus suis adhesin factor H-binding protein (Fhb) targets the host cellular receptor glycolipid GbO3 through its N terminus. However, it is unclear how Fhb interacts with its receptor. Here, we determined the complex structure of factor H-binding protein receptor-binding domain (Fhb RBD) with Gb2, an analog of its receptor, revealing that Gb2 binds in a pocket of the β sandwich core domain. We identified the key residues for Fhb RBD receptor binding using mutagenesis and isothermal titration calorimetry. Mutagenesis analyses indicated that Fhb binds to Gb2 mainly through hydrogen and hydrophobic interactions. Our findings provided structural insights into the Fhb-mediated host-pathogen interactions of S. suis.

PubMed: 27086582
DOI: 10.1002/1873-3468.12174

実験手法

X-RAY DIFFRACTION (2.708 Å)