5BNC

Structure of heme binding protein MSMEG_6519 from Mycobacterium smegmatis

5BNC の概要

• エントリーDOI: 10.2210/pdb5bnc/pdb
• 分子名称: heme binding protein MSMEG_6519, NICKEL (II) ION, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: heme oxygenase, split beta-barrel, heme binding protein
• 由来する生物種: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53769.53

構造登録者

Ahmed, F.H.,Carr, P.D.,Jackson, C.J. (登録日: 2015-05-26, 公開日: 2015-10-21, 最終更新日: 2023-09-27)

主引用文献

Ahmed, F.H.,Carr, P.D.,Lee, B.M.,Afriat-Jurnou, L.,Mohamed, A.E.,Hong, N.S.,Flanagan, J.,Taylor, M.C.,Greening, C.,Jackson, C.J.
Sequence-Structure-Function Classification of a Catalytically Diverse Oxidoreductase Superfamily in Mycobacteria.
J.Mol.Biol., 427:3554-3571, 2015

PubMed Abstract: The deazaflavin cofactor F420 enhances the persistence of mycobacteria during hypoxia, oxidative stress, and antibiotic treatment. However, the identities and functions of the mycobacterial enzymes that utilize F420 under these conditions have yet to be resolved. In this work, we used sequence similarity networks to analyze the distribution of the largest F420-dependent protein family in mycobacteria. We show that these enzymes are part of a larger split β-barrel enzyme superfamily (flavin/deazaflavin oxidoreductases, FDORs) that include previously characterized pyridoxamine/pyridoxine-5'-phosphate oxidases and heme oxygenases. We show that these proteins variously utilize F420, flavin mononucleotide, flavin adenine dinucleotide, and heme cofactors. Functional annotation using phylogenetic, structural, and spectroscopic methods revealed their involvement in heme degradation, biliverdin reduction, fatty acid modification, and quinone reduction. Four novel crystal structures show that plasticity in substrate binding pockets and modifications to cofactor binding motifs enabled FDORs to carry out a variety of functions. This systematic classification and analysis provides a framework for further functional analysis of the roles of FDORs in mycobacterial pathogenesis and persistence.

PubMed: 26434506
DOI: 10.1016/j.jmb.2015.09.021

実験手法

X-RAY DIFFRACTION (2.25 Å)