5B7J

Structure model of Sap1-DNA complex

5B7J の概要

• エントリーDOI: 10.2210/pdb5b7j/pdb
• NMR情報: BMRB: 36001
• 分子名称: Switch-activating protein 1, DNA (5'-D(*CP*AP*AP*AP*AP*CP*AP*AP*TP*AP*TP*T)-3'), DNA (5'-D(*AP*AP*TP*AP*TP*TP*GP*TP*TP*TP*TP*G)-3') (3 entities in total)
• 機能のキーワード: protein, dna, interaction, complex, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• 細胞内の位置: Nucleus : P40847
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 20569.08

構造登録者

Jin, C.,Hu, Y.,Ding, J.,Zhang, Y. (登録日: 2016-06-07, 公開日: 2017-02-22, 最終更新日: 2024-05-01)

主引用文献

Guan, L.,He, P.,Yang, F.,Zhang, Y.,Hu, Y.,Ding, J.,Hua, Y.,Zhang, Y.,Ye, Q.,Hu, J.,Wang, T.,Jin, C.,Kong, D.
Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe
J. Biol. Chem., 292:6056-6075, 2017

PubMed Abstract: A central step in the initiation of chromosomal DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) at late M and early G phase of the cell cycles. Since 1973, four proteins or protein complexes, including cell division control protein 6 (Cdc6)/Cdc18, minichromosome maintenance protein complex, origin recognition complex (ORC), and Cdt1, are known components of the pre-RC. Previously, we reported that a non-ORC protein binds to the essential element Δ9 of the DNA-replication origin ARS3001. In this study, we identified that the non-ORC protein is Sap1. Like ORC, Sap1 binds to DNA origins during cell growth cycles. But unlike ORC, which binds to asymmetric AT-rich sequences through its nine AT-hook motifs, Sap1 preferentially binds to a DNA sequence of 5'-(A/T) (C/G)(A/T)(G/C)(A/T) -3' ( ≥ 1). We also found that Sap1 and ORC physically interact. We further demonstrated that Sap1 is required for the assembly of the pre-RC because of its essential role in recruiting Cdc18 to DNA origins. Thus, we conclude that Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1.

PubMed: 28223353
DOI: 10.1074/jbc.M116.767806

実験手法

SOLUTION NMR