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5B79

Crystal structure of DPF2 double PHD finger

Summary for 5B79
Entry DOI10.2210/pdb5b79/pdb
DescriptorZinc finger protein ubi-d4, ZINC ION (3 entities in total)
Functional Keywordsdpf2, metal binding protein
Biological sourceHomo sapiens (Human)
Cellular locationNucleus: Q92785
Total number of polymer chains1
Total formula weight14080.24
Authors
Li, H.,Xiong, X. (deposition date: 2016-06-05, release date: 2016-10-26, Last modification date: 2023-11-08)
Primary citationXiong, X.,Panchenko, T.,Yang, S.,Zhao, S.,Yan, P.,Zhang, W.,Xie, W.,Li, Y.,Zhao, Y.,Allis, C.D.,Li, H.
Selective recognition of histone crotonylation by double PHD fingers of MOZ and DPF2
Nat.Chem.Biol., 12:1111-1118, 2016
Cited by
PubMed Abstract: Recognition of histone covalent modifications by 'reader' modules constitutes a major mechanism for epigenetic regulation. A recent upsurge of newly discovered histone lysine acylations, such as crotonylation (Kcr), butyrylation (Kbu), and propionylation (Kpr), greatly expands the coding potential of histone lysine modifications. Here we demonstrate that the histone acetylation-binding double PHD finger (DPF) domains of human MOZ (also known as KAT6A) and DPF2 (also known as BAF45d) accommodate a wide range of histone lysine acylations with the strongest preference for Kcr. Crystal structures of the DPF domain of MOZ in complex with H3K14cr, H3K14bu, and H3K14pr peptides reveal that these non-acetyl acylations are anchored in a hydrophobic 'dead-end' pocket with selectivity for crotonylation arising from intimate encapsulation and an amide-sensing hydrogen bonding network. Immunofluorescence and chromatin immunoprecipitation (ChIP)-quantitative PCR (qPCR) showed that MOZ and H3K14cr colocalize in a DPF-dependent manner. Our studies call attention to a new regulatory mechanism centered on histone crotonylation readout by DPF family members.
PubMed: 27775714
DOI: 10.1038/nchembio.2218
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.601 Å)
Structure validation

239149

건을2025-07-23부터공개중

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