5B4S

Crystal Structure of GH80 chitosanase from Mitsuaria chitosanitabida

5B4S の概要

• エントリーDOI: 10.2210/pdb5b4s/pdb
• 分子名称: Chitosanase (2 entities in total)
• 機能のキーワード: chitosanase, glycoside hydrolase, chitosan, hydrolase
• 由来する生物種: Mitsuaria chitosanitabida
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71371.28

構造登録者

Kumasaka, T.,Yorinaga, Y.,Yamamoto, M.,Hamada, K.,Kawamukai, M. (登録日: 2016-04-19, 公開日: 2017-02-01, 最終更新日: 2024-11-13)

主引用文献

Yorinaga, Y.,Kumasaka, T.,Yamamoto, M.,Hamada, K.,Kawamukai, M.
Crystal structure of a family 80 chitosanase from Mitsuaria chitosanitabida
FEBS Lett., 591:540-547, 2017

PubMed Abstract: Chitosanases belong to glycoside hydrolase families 5, 7, 8, 46, 75 and 80 and hydrolyse glucosamine polymers produced by partial or full deacetylation of chitin. Herein, we determined the crystal structure of chitosanase from the β-proteobacterium, Mitsuaria chitosanitabida, (McChoA) at 1.75 Å resolution; the first structure of a family 80 chitosanase. McChoA is a 34 kDa extracellular protein of 301 amino acids that fold into two (upper and lower) globular domains with an active site cleft between them. Key substrate-binding features are conserved with family 24 lysozymes and family 46 chitosanases. The distance between catalytic residues E41 and E61 (10.8 Å) indicates an inverting type mechanism. Uniquely, three disulphide bridges and the C terminus might contribute to enzyme activity.

PubMed: 28084023
DOI: 10.1002/1873-3468.12557

実験手法

X-RAY DIFFRACTION (1.75 Å)