5B3N

The crystal structure of anti-H4K20me1_scFv, 15F11

5B3N の概要

• エントリーDOI: 10.2210/pdb5b3n/pdb
• 分子名称: anti-H4K20me1_scFv (2 entities in total)
• 機能のキーワード: histone modification, scfv, intrabody, immune system
• 由来する生物種: Mus musculus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27246.09

構造登録者

Kujirai, T.,Horikoshi, N.,Kurumizaka, H. (登録日: 2016-03-04, 公開日: 2016-09-21, 最終更新日: 2024-10-16)

主引用文献

Sato, Y.,Kujirai, T.,Arai, R.,Asakawa, H.,Ohtsuki, C.,Horikoshi, N.,Yamagata, K.,Ueda, J.,Nagase, T.,Haraguchi, T.,Hiraoka, Y.,Kimura, A.,Kurumizaka, H.,Kimura, H.
A Genetically Encoded Probe for Live-Cell Imaging of H4K20 Monomethylation
J.Mol.Biol., 428:3885-3902, 2016

PubMed Abstract: Eukaryotic gene expression is regulated in the context of chromatin. Dynamic changes in post-translational histone modification are thought to play key roles in fundamental cellular functions such as regulation of the cell cycle, development, and differentiation. To elucidate the relationship between histone modifications and cellular functions, it is important to monitor the dynamics of modifications in single living cells. A genetically encoded probe called mintbody (modification-specific intracellular antibody), which is a single-chain variable fragment tagged with a fluorescent protein, has been proposed as a useful visualization tool. However, the efficacy of intracellular expression of antibody fragments has been limited, in part due to different environmental conditions in the cytoplasm compared to the endoplasmic reticulum where secreted proteins such as antibodies are folded. In this study, we have developed a new mintbody specific for histone H4 Lys20 monomethylation (H4K20me1). The specificity of the H4K20me1-mintbody in living cells was verified using yeast mutants and mammalian cells in which this target modification was diminished. Expression of the H4K20me1-mintbody allowed us to monitor the oscillation of H4K20me1 levels during the cell cycle. Moreover, dosage-compensated X chromosomes were visualized using the H4K20me1-mintbody in mouse and nematode cells. Using X-ray crystallography and mutational analyses, we identified critical amino acids that contributed to stabilization and/or proper folding of the mintbody. Taken together, these data provide important implications for future studies aimed at developing functional intracellular antibodies. Specifically, the H4K20me1-mintbody provides a powerful tool to track this particular histone modification in living cells and organisms.

PubMed: 27534817
DOI: 10.1016/j.jmb.2016.08.010

実験手法

X-RAY DIFFRACTION (1.94 Å)