5B3G

The crystal structure of the heterodimer of SHORT-ROOT and SCARECROW GRAS domains

5B3G の概要

• エントリーDOI: 10.2210/pdb5b3g/pdb
• 関連するPDBエントリー: 5B3H
• 分子名称: Protein SCARECROW, Protein SHORT-ROOT, PHOSPHATE ION, ... (6 entities in total)
• 機能のキーワード: transcription cofactor, transcription
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95847.50

構造登録者

Hirano, Y.,Nakagawa, M.,Hakoshima, T. (登録日: 2016-02-29, 公開日: 2017-03-01, 最終更新日: 2024-03-20)

主引用文献

Hirano, Y.,Nakagawa, M.,Suyama, T.,Murase, K.,Shirakawa, M.,Takayama, S.,Sun, T.P.,Hakoshima, T.
Structure of the SHR-SCR heterodimer bound to the BIRD/IDD transcriptional factor JKD
Nat Plants, 3:17010-17010, 2017

PubMed Abstract: The plant-specific GAI, RGA and SCR (GRAS) family proteins play critical roles in plant development and signalling. Two GRAS proteins, SHORT-ROOT (SHR) and SCARECROW (SCR), cooperatively direct asymmetric cell division and the patterning of root cell types by transcriptional control in conjunction with BIRD/INDETERMINATE DOMAIN (IDD) transcription factors, although precise details of these specific interactions and actions remain unknown. Here, we present the crystal structures of the SHR-SCR binary and JACKDAW (JKD)/IDD10-SHR-SCR ternary complexes. Each GRAS domain comprises one α/β core subdomain with an α-helical cap that mediates heterodimerization by forming an intermolecular helix bundle. The α/β core subdomain of SHR forms the BIRD binding groove, which specifically recognizes the zinc fingers of JKD. We identified a conserved SHR-binding motif in 13 BIRD/IDD transcription factors. Our results establish a structural basis for GRAS-GRAS and GRAS-BIRD interactions and provide valuable clues towards our understanding of these regulators, which are involved in plant-specific signalling networks.

PubMed: 28211915
DOI: 10.1038/nplants.2017.10

実験手法

X-RAY DIFFRACTION (2 Å)