5B34

Serial Femtosecond Crystallography (SFX) of Ground State Bacteriorhodopsin Crystallized from Bicelles in Complex with Iodine-labeled Detergent HAD13a Determined Using 7-keV X-ray Free Electron Laser (XFEL) at SACLA

Summary for 5B34

• Entry DOI: 10.2210/pdb5b34/pdb
• Related: 5B35
• Descriptor: Bacteriorhodopsin, RETINAL, 2,4,6-tris(iodanyl)-5-(octanoylamino)benzene-1,3-dicarboxylic acid, ... (10 entities in total)
• Functional Keywords: membrane protein, proton pump, transport protein, retinal, sad, proton transport
• Biological source: Halobacterium salinarum
• Cellular location: Cell membrane ; Multi-pass membrane protein : P02945
• Total number of polymer chains: 1
• Total formula weight: 30704.20

Authors

Mizohata, E.,Nakane, T. (deposition date: 2016-02-10, release date: 2016-11-09, Last modification date: 2023-09-06)

• Primary citation: Nakane, T.,Hanashima, S.,Suzuki, M.,Saiki, H.,Hayashi, T.,Kakinouchi, K.,Sugiyama, S.,Kawatake, S.,Matsuoka, S.,Matsumori, N.,Nango, E.,Kobayashi, J.,Shimamura, T.,Kimura, K.,Mori, C.,Kunishima, N.,Sugahara, M.,Takakyu, Y.,Inoue, S.,Masuda, T.,Hosaka, T.,Tono, K.,Joti, Y.,Kameshima, T.,Hatsui, T.,Yabashi, M.,Inoue, T.,Nureki, O.,Iwata, S.,Murata, M.,Mizohata, E.; Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent; Proc.Natl.Acad.Sci.USA, 113:13039-13044, 2016; PubMed: 27799539; DOI: 10.1073/pnas.1602531113

Experimental method

X-RAY DIFFRACTION (2.1 Å)