5B24

The crystal structure of the nucleosome containing cyclobutane pyrimidine dimer

5B24 の概要

• エントリーDOI: 10.2210/pdb5b24/pdb
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (5 entities in total)
• 機能のキーワード: cpd, nucleosome, photolesion, structural protein-dna complex, structural protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 202230.70

構造登録者

Horikoshi, N.,Tachiwana, H.,Kagawa, W.,Osakabe, A.,Kurumizaka, H. (登録日: 2015-12-31, 公開日: 2016-03-02, 最終更新日: 2023-11-08)

主引用文献

Horikoshi, N.,Tachiwana, H.,Kagawa, W.,Osakabe, A.,Matsumoto, S.,Iwai, S.,Sugasawa, K.,Kurumizaka, H.
Crystal structure of the nucleosome containing ultraviolet light-induced cyclobutane pyrimidine dimer
Biochem.Biophys.Res.Commun., 471:117-122, 2016

PubMed Abstract: The cyclobutane pyrimidine dimer (CPD) is induced in genomic DNA by ultraviolet (UV) light. In mammals, this photolesion is primarily induced within nucleosomal DNA, and repaired exclusively by the nucleotide excision repair (NER) pathway. However, the mechanism by which the CPD is accommodated within the nucleosome has remained unknown. We now report the crystal structure of a nucleosome containing CPDs. In the nucleosome, the CPD induces only limited local backbone distortion, and the affected bases are accommodated within the duplex. Interestingly, one of the affected thymine bases is located within 3.0 Å from the undamaged complementary adenine base, suggesting the formation of complementary hydrogen bonds in the nucleosome. We also found that UV-DDB, which binds the CPD at the initial stage of the NER pathway, also efficiently binds to the nucleosomal CPD. These results provide important structural and biochemical information for understanding how the CPD is accommodated and recognized in chromatin.

PubMed: 26837048
DOI: 10.1016/j.bbrc.2016.01.170

実験手法

X-RAY DIFFRACTION (3.6 Å)