5B1M

The mouse nucleosome structure containing H3.1

5B1M の概要

• エントリーDOI: 10.2210/pdb5b1m/pdb
• 関連するPDBエントリー: 5B1L
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1, ... (6 entities in total)
• 機能のキーワード: chromatin, dna binding, histone-fold, structural protein-dna complex, structural protein/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 202410.77

構造登録者

Urahama, T.,Machida, S.,Horikoshi, N.,Osakabe, A.,Tachiwana, H.,Taguchi, H.,Kurumizaka, H. (登録日: 2015-12-08, 公開日: 2017-02-15, 最終更新日: 2023-11-08)

主引用文献

Ueda, J.,Harada, A.,Urahama, T.,Machida, S.,Maehara, K.,Hada, M.,Makino, Y.,Nogami, J.,Horikoshi, N.,Osakabe, A.,Taguchi, H.,Tanaka, H.,Tachiwana, H.,Yao, T.,Yamada, M.,Iwamoto, T.,Isotani, A.,Ikawa, M.,Tachibana, T.,Okada, Y.,Kimura, H.,Ohkawa, Y.,Kurumizaka, H.,Yamagata, K.
Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
Cell Rep, 18:593-600, 2017

PubMed Abstract: Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.

PubMed: 28099840
DOI: 10.1016/j.celrep.2016.12.065

実験手法

X-RAY DIFFRACTION (2.34 Å)