5B0O

Structure of the FliH-FliI complex

5B0O の概要

• エントリーDOI: 10.2210/pdb5b0o/pdb
• 関連するPDBエントリー: 2DPY
• 分子名称: Flagellum-specific ATP synthase, Flagellar assembly protein FliH, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: bacterial flagellum, type iii secretion, atpase, peripheral stalk, hydrolase-motor protein complex, hydrolase/motor protein
• 由来する生物種: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720); 詳細
• 細胞内の位置: Cytoplasm : P26465 P15934
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 321670.54

構造登録者

Imada, K.,Uchida, Y.,Kinoshita, M.,Namba, K.,Minamino, T. (登録日: 2015-11-02, 公開日: 2016-03-23, 最終更新日: 2023-11-08)

主引用文献

Imada, K.,Minamino, T.,Uchida, Y.,Kinoshita, M.,Namba, K.
Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator
Proc.Natl.Acad.Sci.USA, 113:3633-3638, 2016

PubMed Abstract: FliI and FliJ form the FliI6FliJ ATPase complex of the bacterial flagellar export apparatus, a member of the type III secretion system. The FliI6FliJ complex is structurally similar to the α3β3γ complex of F1-ATPase. The FliH homodimer binds to FliI to connect the ATPase complex to the flagellar base, but the details are unknown. Here we report the structure of the homodimer of a C-terminal fragment of FliH (FliHC2) in complex with FliI. FliHC2 shows an unusually asymmetric homodimeric structure that markedly resembles the peripheral stalk of the A/V-type ATPases. The FliHC2-FliI hexamer model reveals that the C-terminal domains of the FliI ATPase face the cell membrane in a way similar to the F/A/V-type ATPases. We discuss the mechanism of flagellar ATPase complex formation and a common origin shared by the type III secretion system and the F/A/V-type ATPases.

PubMed: 26984495
DOI: 10.1073/pnas.1524025113

実験手法

X-RAY DIFFRACTION (3 Å)