5AYW

Structure of a membrane complex

5AYW の概要

• エントリーDOI: 10.2210/pdb5ayw/pdb
• 分子名称: Outer membrane protein assembly factor BamA, Outer membrane protein assembly factor BamB, Outer membrane protein assembly factor BamC, ... (5 entities in total)
• 機能のキーワード: membrane protein, complex, membrane biogenesis
• 由来する生物種: Escherichia coli (strain K12); 詳細
• 細胞内の位置: Cell outer membrane : P0A940; Cell outer membrane ; Lipid-anchor : P77774 P0A903 P0AC02 P0A937
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 172548.46

構造登録者

Huang, Y.,Han, L.,Zheng, J. (登録日: 2015-09-14, 公開日: 2016-02-24, 最終更新日: 2024-03-20)

主引用文献

Han, L.,Zheng, J.,Wang, Y.,Yang, X.,Liu, Y.,Sun, C.,Cao, B.,Zhou, H.,Ni, D.,Lou, J.,Zhao, Y.,Huang, Y.
Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins
Nat.Struct.Mol.Biol., 23:192-196, 2016

PubMed Abstract: In Gram-negative bacteria, the assembly of β-barrel outer-membrane proteins (OMPs) requires the β-barrel-assembly machinery (BAM) complex. We determined the crystal structure of the 200-kDa BAM complex from Escherichia coli at 3.55-Å resolution. The structure revealed that the BAM complex assembles into a hat-like shape, in which the BamA β-barrel domain forms the hat's crown embedded in the outer membrane, and its five polypeptide transport-associated (POTRA) domains interact with the four lipoproteins BamB, BamC, BamD and BamE, thus forming the hat's brim in the periplasm. The assembly of the BAM complex creates a ring-like apparatus beneath the BamA β-barrel in the periplasm and a potential substrate-exit pore located at the outer membrane-periplasm interface. The complex structure suggests that the chaperone-bound OMP substrates may feed into the chamber of the ring-like apparatus and insert into the outer membrane via the potential substrate-exit pore in an energy-independent manner.

PubMed: 26900875
DOI: 10.1038/nsmb.3181

実験手法

X-RAY DIFFRACTION (3.555 Å)