5AWF

Crystal structure of SufB-SufC-SufD complex from Escherichia coli

5AWF の概要

• エントリーDOI: 10.2210/pdb5awf/pdb
• 関連するPDBエントリー: 5AWG
• 分子名称: FeS cluster assembly protein SufB, FeS cluster assembly protein SufD, Probable ATP-dependent transporter SufC (3 entities in total)
• 機能のキーワード: iron-sulfur clusters, iron-sulfur proteins, abc proteins, abc atpase, transport protein-protein binding complex, transport protein/protein binding
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 313822.75

構造登録者

Hirabayashi, K.,Wada, K. (登録日: 2015-07-03, 公開日: 2015-11-11, 最終更新日: 2024-05-29)

主引用文献

Hirabayashi, K.,Yuda, E.,Tanaka, N.,Katayama, S.,Iwasaki, K.,Matsumoto, T.,Kurisu, G.,Outten, F.W.,Fukuyama, K.,Takahashi, Y.,Wada, K.
Functional Dynamics Revealed by the Structure of the SufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis
J.Biol.Chem., 290:29717-29731, 2015

PubMed Abstract: ATP-binding cassette (ABC)-type ATPases are chemomechanical engines involved in diverse biological pathways. Recent genomic information reveals that ABC ATPase domains/subunits act not only in ABC transporters and structural maintenance of chromosome proteins, but also in iron-sulfur (Fe-S) cluster biogenesis. A novel type of ABC protein, the SufBCD complex, functions in the biosynthesis of nascent Fe-S clusters in almost all Eubacteria and Archaea, as well as eukaryotic chloroplasts. In this study, we determined the first crystal structure of the Escherichia coli SufBCD complex, which exhibits the common architecture of ABC proteins: two ABC ATPase components (SufC) with function-specific components (SufB-SufD protomers). Biochemical and physiological analyses based on this structure provided critical insights into Fe-S cluster assembly and revealed a dynamic conformational change driven by ABC ATPase activity. We propose a molecular mechanism for the biogenesis of the Fe-S cluster in the SufBCD complex.

PubMed: 26472926
DOI: 10.1074/jbc.M115.680934

実験手法

X-RAY DIFFRACTION (2.957 Å)