5APN

Structure of the yeast 60S ribosomal subunit in complex with Arx1, Alb1 and N-terminally tagged Rei1

これはPDB形式変換不可エントリーです。

5APN の概要

• エントリーDOI: 10.2210/pdb5apn/pdb
• 関連するPDBエントリー: 5APO
• EMDBエントリー: 3151 3152 3153
• 分子名称: 25S rRNA, 60S ribosomal protein L8-A, 60S ribosomal protein L9-A, ... (49 entities in total)
• 機能のキーワード: ribosome, eukaryotic ribosome biogenesis, ribosome maturation, ribosome biogenesis factor, 60s ribosomal subunit, rei1, arx1, alb1, cryo-em, ribosomal polypeptide exit tunnel proofreading
• 由来する生物種: Saccharomyces cerevisiae S288c; 詳細
• タンパク質・核酸の鎖数: 47
• 化学式量合計: 2088780.95

構造登録者

Greber, B.J.,Gerhardy, S.,Leitner, A.,Leibundgut, M.,Salem, M.,Boehringer, D.,Leulliot, N.,Aebersold, R.,Panse, V.G.,Ban, V. (登録日: 2015-09-17, 公開日: 2015-12-16, 最終更新日: 2024-10-16)

主引用文献

Greber, B.J.,Gerhardy, S.,Leitner, A.,Leibundgut, M.,Salem, M.,Boehringer, D.,Leulliot, N.,Aebersold, R.,Panse, V.G.,Ban, N.
Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.
Cell, 164:91-, 2015

PubMed Abstract: Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the proofreading of functional centers of the 60S subunit and the release of several ribosome biogenesis factors. We report the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution. In addition to the network of interactions formed by Alb1, the structure reveals a mechanism for ensuring the integrity of the ribosomal polypeptide exit tunnel. Arx1 probes the entire set of inner-ring proteins surrounding the tunnel exit, and the C terminus of Rei1 is deeply inserted into the ribosomal tunnel, where it forms specific contacts along almost its entire length. We provide genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation.

PubMed: 26709046
DOI: 10.1016/J.CELL.2015.11.027

実験手法

ELECTRON MICROSCOPY (3.91 Å)