5AOO
X-ray structure of a human Kobuvirus: Aichi virus A (AiV)
Summary for 5AOO
| Entry DOI | 10.2210/pdb5aoo/pdb |
| Descriptor | VP0, VP1, VP3, ... (4 entities in total) |
| Functional Keywords | virus, aichi virus, kobuvirus, picornavirus, acute gastroenterisis, icosahedral capsid, full virus particle |
| Biological source | AICHIVIRUS A More |
| Cellular location | Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Virion : Q91QP4 Q91QP4 Q91QP4 |
| Total number of polymer chains | 3 |
| Total formula weight | 90227.69 |
| Authors | Sabin, C.,Palkova, L.,Plevka, P. (deposition date: 2015-09-11, release date: 2016-03-09, Last modification date: 2024-01-10) |
| Primary citation | Sabin, C.,Plevka, P. The Use of Noncrystallographic Symmetry Averaging to Solve Structures from Data Affected by Perfect Hemihedral Twinning Acta Crystallogr.,Sect.F, 72:188-, 2016 Cited by PubMed Abstract: Hemihedral twinning is a crystal-growth anomaly in which a specimen is composed of two crystal domains that coincide with each other in three dimensions. However, the orientations of the crystal lattices in the two domains differ in a specific way. In diffraction data collected from hemihedrally twinned crystals, each observed intensity contains contributions from both of the domains. With perfect hemihedral twinning, the two domains have the same volumes and the observed intensities do not contain sufficient information to detwin the data. Here, the use of molecular replacement and of noncrystallographic symmetry (NCS) averaging to detwin a 2.1 Å resolution data set for Aichi virus 1 affected by perfect hemihedral twinning is described. The NCS averaging enabled the correction of errors in the detwinning introduced by the differences between the molecular-replacement model and the crystallized structure. The procedure permitted the structure to be determined from a molecular-replacement model that had 16% sequence identity and a 1.6 Å r.m.s.d. for C(α) atoms in comparison to the crystallized structure. The same approach could be used to solve other data sets affected by perfect hemihedral twinning from crystals with NCS. PubMed: 26919522DOI: 10.1107/S2053230X16000923 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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