5AOO

X-ray structure of a human Kobuvirus: Aichi virus A (AiV)

5AOO の概要

• エントリーDOI: 10.2210/pdb5aoo/pdb
• 分子名称: VP0, VP1, VP3, ... (4 entities in total)
• 機能のキーワード: virus, aichi virus, kobuvirus, picornavirus, acute gastroenterisis, icosahedral capsid, full virus particle
• 由来する生物種: AICHIVIRUS A; 詳細
• 細胞内の位置: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Virion : Q91QP4 Q91QP4 Q91QP4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 90227.69

構造登録者

Sabin, C.,Palkova, L.,Plevka, P. (登録日: 2015-09-11, 公開日: 2016-03-09, 最終更新日: 2024-01-10)

主引用文献

Sabin, C.,Plevka, P.
The Use of Noncrystallographic Symmetry Averaging to Solve Structures from Data Affected by Perfect Hemihedral Twinning
Acta Crystallogr.,Sect.F, 72:188-, 2016

PubMed Abstract: Hemihedral twinning is a crystal-growth anomaly in which a specimen is composed of two crystal domains that coincide with each other in three dimensions. However, the orientations of the crystal lattices in the two domains differ in a specific way. In diffraction data collected from hemihedrally twinned crystals, each observed intensity contains contributions from both of the domains. With perfect hemihedral twinning, the two domains have the same volumes and the observed intensities do not contain sufficient information to detwin the data. Here, the use of molecular replacement and of noncrystallographic symmetry (NCS) averaging to detwin a 2.1 Å resolution data set for Aichi virus 1 affected by perfect hemihedral twinning is described. The NCS averaging enabled the correction of errors in the detwinning introduced by the differences between the molecular-replacement model and the crystallized structure. The procedure permitted the structure to be determined from a molecular-replacement model that had 16% sequence identity and a 1.6 Å r.m.s.d. for C(α) atoms in comparison to the crystallized structure. The same approach could be used to solve other data sets affected by perfect hemihedral twinning from crystals with NCS.

PubMed: 26919522
DOI: 10.1107/S2053230X16000923

実験手法

X-RAY DIFFRACTION (2.1 Å)