5AMW

Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 2 protein (A141F) processed with the CrystalDirect automated mounting and cryo-cooling technology

5AMW の概要

• エントリーDOI: 10.2210/pdb5amw/pdb
• 関連するPDBエントリー: 5AMX 5AMY 5AMZ 5AN2 5AN4 5AND 5ANE 5ANG 5ANI 5ANJ 5ANK 5ANL 5ANO
• 分子名称: Fra a 2 allergen (2 entities in total)
• 機能のキーワード: allergen, pyr/pyl/rcar, bet v 1, flavonoids, automated crystal harvesting, automated cryo-cooling, crystaldirect
• 由来する生物種: Fragaria ananassa (Strawberry)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35478.14

構造登録者

Zander, U.,Casanal, A.,Pott, D.,Valpuesta, V.,Hoffmann, G.,Cornaciu, I.,Cipriani, F.,Marquez, J.A. (登録日: 2015-09-02, 公開日: 2016-04-13, 最終更新日: 2024-05-08)

主引用文献

Zander, U.,Hoffmann, G.,Cornaciu, I.,Marquette, J.-P.,Papp, G.,Landret, C.,Seroul, G.,Sinoir, J.,Roewer, M.,Felisaz, F.,Rodriguez-Puente, S.,Mariaule, V.,Murphy, P.,Mathieu, M.,Cipriani, F.,Marquez, J.A.
Automated Harvesting and Processing of Protein Crystals Through Laser Photoablation.
Acta Crystallogr.,Sect.D, 72:454-, 2016

PubMed Abstract: Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting and processing largely depend on manual operations. Here, a series of new methods are presented based on the use of a low X-ray-background film as a crystallization support and a photoablation laser that enable the automation of major operations required for the preparation of crystals for X-ray diffraction experiments. In this approach, the controlled removal of the mother liquor before crystal mounting simplifies the cryocooling process, in many cases eliminating the use of cryoprotectant agents, while crystal-soaking experiments are performed through diffusion, precluding the need for repeated sample-recovery and transfer operations. Moreover, the high-precision laser enables new mounting strategies that are not accessible through other methods. This approach bridges an important gap in automation and can contribute to expanding the capabilities of modern macromolecular crystallography facilities.

PubMed: 27050125
DOI: 10.1107/S2059798316000954

実験手法

X-RAY DIFFRACTION (1.9 Å)