5AJU

Crystal structure of ligand-free phosphoribohydroxylase lonely guy from Claviceps purpurea in complex with phosphoribose

5AJU の概要

• エントリーDOI: 10.2210/pdb5aju/pdb
• 関連するPDBエントリー: 5AJT
• 分子名称: PHOSPHORIBOHYDROLASE LONELY GUY, 5-O-phosphono-beta-D-ribofuranose, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, cytokinin, rossman fold, protein lonely guy, claviceps purpurea, lysine decarboxylase, phosphoribohydrolase
• 由来する生物種: CLAVICEPS PURPUREA (ERGOT FUNGUS)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29498.10

構造登録者

Dzurova, L.,Savino, S.,Forneris, F. (登録日: 2015-02-27, 公開日: 2015-06-03, 最終更新日: 2024-01-10)

主引用文献

Dzurova, L.,Forneris, F.,Savino, S.,Galuszka, P.,Vrabka, J.,Frebort, I.
The Three-Dimensional Structure of "Lonely Guy" from Claviceps Purpurea Provides Insights Into the Phosphoribohydrolase Function of Rossmann Fold-Containing Lysine Decarboxylase-Like Proteins.
Proteins, 83:1539-, 2015

PubMed Abstract: The recently discovered cytokinin (CK)-specific phosphoribohydrolase "Lonely Guy" (LOG) is a key enzyme of CK biosynthesis, converting inactive CK nucleotides into biologically active free bases. We have determined the crystal structures of LOG from Claviceps purpurea (cpLOG) and its complex with the enzymatic product phosphoribose. The structures reveal a dimeric arrangement of Rossmann folds, with the ligands bound to large pockets at the interface between cpLOG monomers. Structural comparisons highlight the homology of cpLOG to putative lysine decarboxylases. Extended sequence analysis enabled identification of a distinguishing LOG sequence signature. Taken together, our data suggest phosphoribohydrolase activity for several proteins of unknown function.

PubMed: 26010010
DOI: 10.1002/PROT.24835

実験手法

X-RAY DIFFRACTION (2.5 Å)