5AJ4

Structure of the 55S mammalian mitoribosome.

これはPDB形式変換不可エントリーです。

5AJ4 の概要

• エントリーDOI: 10.2210/pdb5aj4/pdb
• 関連するPDBエントリー: 4V19 4V1A 5AJ3
• EMDBエントリー: 2914
• 分子名称: MITORIBOSOMAL 12S Ribosomal RNA, MITORIBOSOMAL PROTEIN US12M, MRPS12, MITORIBOSOMAL PROTEIN US14M, MRPS14, ... (92 entities in total)
• 機能のキーワード: ribosome, translation, mitochondria, mammalian 55s mitoribosome, mammalian 55s mitochondrial ribosome, 28s small subunit, 39s large subunit, mrna, trna, ptc, decoding center, cryo- em, single particle analysis
• 由来する生物種: SUS SCROFA (PIG); 詳細
• タンパク質・核酸の鎖数: 89
• 化学式量合計: 2758859.31

構造登録者

Greber, B.J.,Bieri, P.,Leibundgut, M.,Leitner, A.,Aebersold, R.,Boehringer, D.,Ban, N. (登録日: 2015-02-20, 公開日: 2015-04-22, 最終更新日: 2024-10-23)

主引用文献

Greber, B.J.,Bieri, P.,Leibundgut, M.,Leitner, A.,Aebersold, R.,Boehringer, D.,Ban, N.
The complete structure of the 55S mammalian mitochondrial ribosome.
Science, 348:303-308, 2015

PubMed Abstract: Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 angstrom resolution by cryo-electron microscopy and chemical cross-linking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 angstrom resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA (mRNA) binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.

PubMed: 25837512
DOI: 10.1126/science.aaa3872

実験手法

ELECTRON MICROSCOPY (3.8 Å)