5AHN

IMP-bound form of the D199N mutant of IMPDH from Pseudomonas aeruginosa

5AHN の概要

• エントリーDOI: 10.2210/pdb5ahn/pdb
• 関連するPDBエントリー: 5AHL 5AHM
• 分子名称: INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE, INOSINIC ACID, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, cbs module, point mutant, allosteric regulation nucleotide metabolism
• 由来する生物種: PSEUDOMONAS AERUGINOSA PAO1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52163.19

構造登録者

Labesse, G.,Alexandre, T.,Gelin, M.,Haouz, A.,Munier-Lehmann, H. (登録日: 2015-02-06, 公開日: 2015-07-15, 最終更新日: 2024-01-10)

主引用文献

Labesse, G.,Alexandre, T.,Gelin, M.,Haouz, A.,Munier-Lehmann, H.
Crystallographic Studies of Two Variants of Pseudomonas Aeruginosa Impdh with Impaired Allosteric Regulation
Acta Crystallogr.,Sect.D, 71:1890-, 2015

PubMed Abstract: Inosine-5'-monophosphate dehydrogenases (IMPDHs), which are the rate-limiting enzymes in guanosine-nucleotide biosynthesis, are important therapeutic targets. Despite in-depth functional and structural characterizations of various IMPDHs, the role of the Bateman domain containing two CBS motifs remains controversial. Their involvement in the allosteric regulation of Pseudomonas aeruginosa IMPDH by Mg-ATP has recently been reported. To better understand the function of IMPDH and the importance of the CBS motifs, the structure of a variant devoid of these modules (ΔCBS) was solved at high resolution in the apo form and in complex with IMP. In addition, a single amino-acid substitution variant, D199N, was also structurally characterized: the mutation corresponds to the autosomal dominant mutant D226N of human IMPDH1, which is responsible for the onset of the retinopathy adRP10. These new structures shed light onto the possible mechanism of regulation of the IMPDH enzymatic activity. In particular, three conserved loops seem to be key players in this regulation as they connect the tetramer-tetramer interface with the active site and show significant modification upon substrate binding.

PubMed: 26327379
DOI: 10.1107/S1399004715013115

実験手法

X-RAY DIFFRACTION (1.652 Å)