5AEC

Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.

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5AEC の概要

• エントリーDOI: 10.2210/pdb5aec/pdb
• 分子名称: 3,6-DIKETOCAMPHANE 1,6 MONOOXYGENASE, PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID), GLYCEROL, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, biocatalysis, flavin monooxygenase
• 由来する生物種: PSEUDOMONAS PUTIDA
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86869.51

構造登録者

Isupov, M.N.,Schroeder, E.,Gibson, R.P.,Beecher, J.,Donadio, G.,Saneei, V.,Dcunha, S.,McGhie, E.J.,Sayer, C.,Davenport, C.F.,Lau, P.C.,Hasegawa, Y.,Iwaki, H.,Kadow, M.,Loschinski, K.,Bornscheuer, U.T.,Bourenkov, G.,Littlechild, J.A. (登録日: 2015-08-28, 公開日: 2015-09-09, 最終更新日: 2024-01-10)

主引用文献

Isupov, M.N.,Schroder, E.,Gibson, R.P.,Beecher, J.,Donadio, G.,Saneei, V.,Dcunha, S.A.,Mcghie, E.J.,Sayer, C.,Davenport, C.F.,Lau, P.C.,Hasegawa, Y.,Iwaki, H.,Kadow, M.,Balke, K.,Bornscheuer, U.T.,Bourenkov, G.,Littlechild, J.A.
The Oxygenating Constituent of 3,6-Diketocamphane Monooxygenase from the Cam Plasmid of Pseudomonas Putida: The First Crystal Structure of a Type II Baeyer-Villiger Monooxygenase.
Acta Crystallogr.,Sect.D, 71:2344-, 2015

PubMed Abstract: The three-dimensional structures of the native enzyme and the FMN complex of the overexpressed form of the oxygenating component of the type II Baeyer-Villiger 3,6-diketocamphane monooxygenase have been determined to 1.9 Å resolution. The structure of this dimeric FMN-dependent enzyme, which is encoded on the large CAM plasmid of Pseudomonas putida, has been solved by a combination of multiple anomalous dispersion from a bromine crystal soak and molecular replacement using a bacterial luciferase model. The orientation of the isoalloxazine ring of the FMN cofactor in the active site of this TIM-barrel fold enzyme differs significantly from that previously observed in enzymes of the bacterial luciferase-like superfamily. The Ala77 residue is in a cis conformation and forms a β-bulge at the C-terminus of β-strand 3, which is a feature observed in many proteins of this superfamily.

PubMed: 26527149
DOI: 10.1107/S1399004715017939

実験手法

X-RAY DIFFRACTION (1.93 Å)