5ADY

Cryo-EM structures of the 50S ribosome subunit bound with HflX

Summary for 5ADY

Related1B75 1D6K 1DFU 2J28 2VRH 487D 4CSU 4UY8 5AKA
EMDB information3133
Descriptor50S RIBOSOMAL PROTEIN L32, 50S RIBOSOMAL PROTEIN L33, 50S RIBOSOMAL PROTEIN L34, ... (36 entities in total)
Functional Keywordsribosome, ribosome rescue
Biological sourceESCHERICHIA COLI K-12
Total number of polymer chains34
Total molecular weight1448692.51
Authors
Zhang, Y.,Mandava, C.S.,Cao, W.,Li, X.,Zhang, D.,Li, N.,Zhang, Y.,Zhang, X.,Qin, Y.,Mi, K.,Lei, J.,Sanyal, S.,Gao, N. (deposition date: 2015-08-25, release date: 2015-10-14, Last modification date: 2018-10-24)
Primary citation
Zhang, Y.,Mandava, C.S.,Cao, W.,Li, X.,Zhang, D.,Li, N.,Zhang, Y.,Zhang, X.,Qin, Y.,Mi, K.,Lei, J.,Sanyal, S.,Gao, N.
Hflx is a Ribosome Splitting Factor Rescuing Stalled Ribosomes Under Stress Conditions
Nat.Struct.Mol.Biol., 22:906-, 2015
PubMed: 26458047 (PDB entries with the same primary citation)
DOI: 10.1038/NSMB.3103
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (4.5 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone57 6.7% 0.2% 0.35MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5ady
no rotation
Molmil generated image of 5ady
rotated about x axis by 90°
Molmil generated image of 5ady
rotated about y axis by 90°