5ADX

CryoEM structure of dynactin complex at 4.0 angstrom resolution

「5AFT」から置き換えられました

5ADX の概要

• エントリーDOI: 10.2210/pdb5adx/pdb
• EMDBエントリー: 2856 2857
• 分子名称: ACTIN RELATED PROTEIN 1, DYNACTIN 5, DYNACTIN SUBUNIT 6, ... (17 entities in total)
• 機能のキーワード: structural protein, dynein co-factor, actin-like filament, cellular cargo transport
• 由来する生物種: SUS SCROFA (PIG); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton: Q6QAQ1
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 805390.81

構造登録者

Zhang, K.,Urnavicius, L.,Diamant, A.G.,Motz, C.,Schlage, M.A.,Yu, M.,Patel, N.A.,Robinson, C.V.,Carter, A.P. (登録日: 2015-08-24, 公開日: 2015-12-30, 最終更新日: 2024-05-08)

主引用文献

Urnavicius, L.,Zhang, K.,Diamant, A.G.,Motz, C.,Schlager, M.A.,Yu, M.,Patel, N.A.,Robinson, C.V.,Carter, A.P.
The Structure of the Dynactin Complex and its Interaction with Dynein.
Science, 347:1441-, 2015

PubMed Abstract: Dynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our reconstruction reveals how dynactin is built around a filament containing eight copies of the actin-related protein Arp1 and one of β-actin. The filament is capped at each end by distinct protein complexes, and its length is defined by elongated peptides that emerge from the α-helical shoulder domain. A further 8.2 angstrom structure of the complex between dynein, dynactin, and the motility-inducing cargo adaptor Bicaudal-D2 shows how the translational symmetry of the dynein tail matches that of the dynactin filament. The Bicaudal-D2 coiled coil runs between dynein and dynactin to stabilize the mutually dependent interactions between all three components.

PubMed: 25814576
DOI: 10.1126/SCIENCE.AAA4080

実験手法

ELECTRON MICROSCOPY (4 Å)