5AAO

Crystal structure of fluorogen-activating designed ankyrin repeat protein (DARPin) dimer in complex with malachite green

Summary for 5AAO

Entry DOI	10.2210/pdb5aao/pdb
Descriptor	FAD3210, 4-[[4-(dimethylamino)cyclohexa-2,5-dien-1-ylidene]-(4-methoxyphenyl)methyl]-N,N-dimethyl-aniline (3 entities in total)
Functional Keywords	fluorescent protein, homodimer, designed ankyrin repeat protein (darpin), fluorogen-activation, malachite green
Biological source	SYNTHETIC CONSTRUCT
Total number of polymer chains	12
Total formula weight	224784.14
Authors	Batyuk, A.,Schuetz, M.,Kummer, L.,Wu, Y.,Mittl, P.,Plueckthun, A. (deposition date: 2015-07-27, release date: 2016-02-03, Last modification date: 2024-01-10)
Primary citation	Schutz, M.,Batyuk, A.,Klenk, C.,Kummer, L.,de Picciotto, S.,Gulbakan, B.,Wu, Y.,Newby, G.A.,Zosel, F.,Schoppe, J.,Sedlak, E.,Mittl, P.R.,Zenobi, R.,Wittrup, K.D.,Pluckthun, A. Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools. J. Mol. Biol., 428:1272-1289, 2016 Cited by PubMed: 26812208 DOI: 10.1016/j.jmb.2016.01.017 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation