5A7A

Novel inter-subunit contacts in Barley Stripe Mosaic Virus revealed by cryo-EM

Summary for 5A7A

• Entry DOI: 10.2210/pdb5a7a/pdb
• Related: 5A79
• EMDB information: 3074
• Descriptor: BARLEY STRIPE MOSAIC VIRUS NARROW, RNA (2 entities in total)
• Functional Keywords: virus, bsmv, helical, cryo-em, image processing, msa
• Biological source: BARLEY STRIPE MOSAIC VIRUS; More
• Cellular location: Virion : P04866
• Total number of polymer chains: 2
• Total formula weight: 23513.88

Authors

Clare, D.K.,Pechnikova, E.,Skurat, E.,Makarov, V.,Sokolova, O.S.,Solovyev, A.G.,V Orlova, E. (deposition date: 2015-07-03, release date: 2015-09-02, Last modification date: 2024-05-08)

Primary citation

Clare, D.K.,Pechnikova, E.V.,Skurat, E.V.,Makarov, V.V.,Sokolova, O.S.,Solovyev, A.G.,Orlova, E.V.
Novel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy.
Structure, 23:1815-, 2015

PubMed Abstract: Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report the first high-resolution structure of BSMV at 4.1 Å obtained by cryo-electron microscopy. We discovered that BSMV forms two types of virion that differ in the number of coat protein (CP) subunits per turn and interactions between the CP subunits. While BSMV and tobacco mosaic virus CP subunits have a similar fold and interact with RNA using conserved residues, the axial contacts between the CP of these two viral groups are considerably different. BSMV CP subunits lack substantial axial contacts and are held together by a previously unobserved lateral contact formed at the virion surface via an interacting loop, which protrudes from the CP hydrophobic core to the adjacent CP subunit. These data provide an insight into diversity in structural organization of helical viruses.

PubMed: 26278173
DOI: 10.1016/J.STR.2015.06.028

Experimental method

ELECTRON MICROSCOPY (4.1 Å)