5A6R

Crystal structure of the BTB domain of human KCTD17

5A6R の概要

• エントリーDOI: 10.2210/pdb5a6r/pdb
• 分子名称: BTB/POZ DOMAIN-CONTAINING PROTEIN KCTD17 (2 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm : Q8N5Z5
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 76851.71

構造登録者

Pinkas, D.M.,Sorrell, F.,Sanvitale, C.E.,Goubin, S.,Williams, E.,Newman, J.A.,Pearce, N.M.,Neshich, I.,Pike, A.C.W.,MacKenzie, A.,Quigley, A.,Faust, B.,Carpenter, E.P.,Tallant, C.,Kopec, J.,Chalk, R.,Krojer, T.,Burgess-Brown, N.A.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,Bullock, A. (登録日: 2015-06-30, 公開日: 2015-11-04, 最終更新日: 2024-01-10)

主引用文献

Pinkas, D.M.,Sanvitale, C.E.,Bufton, J.C.,Sorrell, F.J.,Solcan, N.,Chalk, R.,Doutch, J.,Bullock, A.N.
Structural complexity in the KCTD family of Cullin3-dependent E3 ubiquitin ligases.
Biochem. J., 474:3747-3761, 2017

PubMed Abstract: Members of the potassium channel tetramerization domain (KCTD) family are soluble non-channel proteins that commonly function as Cullin3 (Cul3)-dependent E3 ligases. Solution studies of the N-terminal BTB domain have suggested that some KCTD family members may tetramerize similarly to the homologous tetramerization domain (T1) of the voltage-gated potassium (Kv) channels. However, available structures of KCTD1, KCTD5 and KCTD9 have demonstrated instead pentameric assemblies. To explore other phylogenetic clades within the KCTD family, we determined the crystal structures of the BTB domains of a further five human KCTD proteins revealing a rich variety of oligomerization architectures, including monomer (SHKBP1), a novel two-fold symmetric tetramer (KCTD10 and KCTD13), open pentamer (KCTD16) and closed pentamer (KCTD17). While these diverse geometries were confirmed by small-angle X-ray scattering (SAXS), only the pentameric forms were stable upon size-exclusion chromatography. With the exception of KCTD16, all proteins bound to Cul3 and were observed to reassemble in solution as 5 : 5 heterodecamers. SAXS data and structural modelling indicate that Cul3 may stabilize closed BTB pentamers by binding across their BTB-BTB interfaces. These extra interactions likely also allow KCTD proteins to bind Cul3 without the expected 3-box motif. Overall, these studies reveal the KCTD family BTB domain to be a highly versatile scaffold compatible with a range of oligomeric assemblies and geometries. This observed interface plasticity may support functional changes in regulation of this unusual E3 ligase family.

PubMed: 28963344
DOI: 10.1042/BCJ20170527

実験手法

X-RAY DIFFRACTION (2.85 Å)