5A6D

Proliferating Cell Nuclear Antigen, PCNA, from Thermococcus gammatolerans

5A6D の概要

• エントリーDOI: 10.2210/pdb5a6d/pdb
• 分子名称: DNA POLYMERASE SLIDING CLAMP (1 entity in total)
• 機能のキーワード: nuclear protein, dna binding protein, pcna, thermococcus, gammatolerans, proliferating, nuclear antigen.
• 由来する生物種: THERMOCOCCUS GAMMATOLERANS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56312.46

構造登録者

Venancio-Landeros, A.,Cardona-Felix, C.S.,Rudino-Pinera, E. (登録日: 2015-06-25, 公開日: 2016-07-06, 最終更新日: 2024-01-10)

主引用文献

Venancio-Landeros, A.A.,Rudino-Pinera, E.,Cardona-Felix, C.S.
Cloning, recombinant production and crystallographic structure of Proliferating Cell Nuclear Antigen from radioresistant archaeon Thermococcus gammatolerans.
Biochem Biophys Rep, 8:200-206, 2016

PubMed Abstract: is a strictly anaerobic; hyperthermophilicarchaeon belongs to the order in the phylum . It was extracted from a hydrothermal vent from the Guaymas Basin (Gulf of California, Mexico). Different studies show that is one of the most radioresistant organisms known amongst the . This makes it a unique model to study adaptations to the environment and to study DNA repair mechanisms in an organism able to tolerate harsh conditions. A key protein in these mechanisms is the Proliferation Cell Nuclear Antigen (PCNA). Its function is focused on their ability to slide along the DNA duplex and coordinating the activities of proteins mainly related to DNA edition and processing. Analysis of proteins have proven to be enormously fruitful because much of the information obtained from them can be extrapolated to eukaryotic systems, and PCNA is no exception. Here we report the cloning, recombinant expression and crystallographic structure of PCNA from (TgPCNA).

PubMed: 28955957
DOI: 10.1016/j.bbrep.2016.08.004

実験手法

X-RAY DIFFRACTION (2.8 Å)