5A4O

Crystal structure of BPSL1147, a PC4 homolog from Burkholderia pseudomallei K96243 (orthorhombic crystal form)

5A4O の概要

• エントリーDOI: 10.2210/pdb5a4o/pdb
• 関連するPDBエントリー: 5A4N
• 分子名称: BPSL1147, CHLORIDE ION (3 entities in total)
• 機能のキーワード: dna-binding protein, ssdna-binding protein, dna replication, recombination and repair, dna binding protein
• 由来する生物種: BURKHOLDERIA PSEUDOMALLEI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17324.31

構造登録者

Werten, S.,Bayer, N.,Hinrichs, W. (登録日: 2015-06-11, 公開日: 2016-04-06, 最終更新日: 2024-05-01)

主引用文献

Werten, S.,Kohler, C.,Bayer, N.,Steinmetz, I.,Hinrichs, W.
Structural Analysis and Knock-Out of a Burkholderia Pseudomallei Homolog of the Eukaryotic Transcription Coactivator Pc4.
Gene, 557:140-, 2016

PubMed Abstract: Homologs of the eukaryotic transcription coactivator PC4, which also functions in DNA repair and oxidative stress, were recently identified in prokaryotes. Crystallographic analysis of BPSL1147, a putative homolog from the pathogen Burkholderia pseudomallei K96243, reveals a highly conserved core structure and suggests a nucleic acid binding mode similar to that of PC4. Knock-out and complementation experiments do not reveal distinguishing phenotypes under normal growth conditions or in the presence of H2O2, arguing against a critical role in repair or the oxidative stress response of Burkholderia. These results may reflect redundancy or point at a bacteriophage origin of Burkholderia PC4 homologs.

PubMed: 26625975
DOI: 10.1016/J.GENE.2015.11.037

実験手法

X-RAY DIFFRACTION (1.76 Å)