5A3E

2.5A structure of lysozyme determined by MicroED with data from a single crystal

Summary for 5A3E

Entry DOI	10.2210/pdb5a3e/pdb
EMDB information	6342
Descriptor	LYSOZYME C (2 entities in total)
Functional Keywords	microed, hydrolase
Biological source	GALLUS GALLUS (CHICKEN)
Cellular location	Secreted: P00698
Total number of polymer chains	1
Total formula weight	14331.16
Authors	Nannenga, B.L.,Shi, D.,Leslie, A.G.W.,Gonen, T. (deposition date: 2015-05-28, release date: 2015-06-17, Last modification date: 2024-11-13)
Primary citation	Nannenga, B.L.,Shi, D.,Leslie, A.G.W.,Gonen, T. High-Resolution Structure Determination by Continuous-Rotation Data Collection in Microed. Nat.Methods, 11:927-, 2014 Cited by PubMed Abstract: MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology. PubMed: 25086503 DOI: 10.1038/NMETH.3043 PDB entries with the same primary citation
Experimental method	ELECTRON CRYSTALLOGRAPHY (2.501 Å)

Structure validation