5A3E

2.5A structure of lysozyme determined by MicroED with data from a single crystal

5A3E の概要

• エントリーDOI: 10.2210/pdb5a3e/pdb
• EMDBエントリー: 6342
• 分子名称: LYSOZYME C (2 entities in total)
• 機能のキーワード: microed, hydrolase
• 由来する生物種: GALLUS GALLUS (CHICKEN)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14331.16

構造登録者

Nannenga, B.L.,Shi, D.,Leslie, A.G.W.,Gonen, T. (登録日: 2015-05-28, 公開日: 2015-06-17, 最終更新日: 2024-11-13)

主引用文献

Nannenga, B.L.,Shi, D.,Leslie, A.G.W.,Gonen, T.
High-Resolution Structure Determination by Continuous-Rotation Data Collection in Microed.
Nat.Methods, 11:927-, 2014

PubMed Abstract: MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.

PubMed: 25086503
DOI: 10.1038/NMETH.3043

実験手法

ELECTRON CRYSTALLOGRAPHY (2.501 Å)