5A2H

Crystal Structure of Arabidopsis thaliana Calmodulin-7

5A2H の概要

• エントリーDOI: 10.2210/pdb5a2h/pdb
• 分子名称: CALMODULIN, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• 機能のキーワード: calcium-binding protein, protein
• 由来する生物種: ARABIDOPSIS THALIANA (MOUSE-EAR CRESS, THALE-CRESS)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17437.51

構造登録者

Kumar, S.,Gourinath, S. (登録日: 2015-05-19, 公開日: 2016-03-30, 最終更新日: 2024-01-10)

主引用文献

Kumar, S.,Mazumder, M.,Gupta, N.,Chattopadhyay, S.,Gourinath, S.
Crystal Structure of Arabidopsis Thaliana Calmodulin7 and Insight Into its Mode of DNA Binding.
FEBS Lett., 590:3029-, 2016

PubMed Abstract: Calmodulin (CaM) is a Ca(2+) sensor that participates in several cellular signaling cascades by interacting with various targets, including DNA. It has been shown that Arabidopsis thaliana CaM7 (AtCaM7) interacts with Z-box DNA and functions as a transcription factor [Kushwaha R et al. (2008) Plant Cell 20, 1747-1759; Abbas N et al. (2014) Plant Cell 26, 1036-1052]. The crystal structure of AtCaM7, and a model of the AtCAM7-Z-box complex suggest that Arg-127 determines the DNA-binding ability by forming crucial interactions with the guanine base. We validated the model using biolayer interferometry, which confirmed that AtCaM7 interacts with Z-box DNA with high affinity. In contrast, the AtCaM2/3/5 isoform does not show any binding, although it differs from AtCaM7 by only a single residue.

PubMed: 27500568
DOI: 10.1002/1873-3468.12349

実験手法

X-RAY DIFFRACTION (2.27 Å)