5A1V

The structure of the COPI coat linkage I

5A1V の概要

• エントリーDOI: 10.2210/pdb5a1v/pdb
• 関連するPDBエントリー: 5A1U 5A1W 5A1X 5A1Y
• EMDBエントリー: 2986
• 分子名称: ADP-RIBOSYLATION FACTOR 1, COATOMER SUBUNIT ALPHA, COATOMER SUBUNIT BETA, ... (8 entities in total)
• 機能のキーワード: transport protein, copi, coatomer, coated vesicles
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• 細胞内の位置: Golgi apparatus: P11076; Cytoplasm . Xenin: Secreted : Q8CIE6; Cytoplasm, cytosol : O55029 Q9QZE5; Cytoplasm : P61924 Q9JIF7 Q5XJY5 O89079
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 1780700.91

構造登録者

Dodonova, S.O.,Diestelkoetter-Bachert, P.,von Appen, A.,Hagen, W.J.H.,Beck, R.,Beck, M.,Wieland, F.,Briggs, J.A.G. (登録日: 2015-05-06, 公開日: 2015-07-08, 最終更新日: 2024-05-08)

主引用文献

Dodonova, S.O.,Diestelkoetter-Bachert, P.,Von Appen, A.,Hagen, W.J.H.,Beck, R.,Beck, M.,Wieland, F.,Briggs, J.A.G.
Vesicular Transport. A Structure of the Copi Coat and the Role of Coat Proteins in Membrane Vesicle Assembly.
Science, 349:195-, 2015

PubMed Abstract: Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.

PubMed: 26160949
DOI: 10.1126/SCIENCE.AAB1121

実験手法

ELECTRON MICROSCOPY (21 Å)