5X7H

Crystal Structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase complexed with cycloisomaltoheptaose

Summary for 5X7H

• Entry DOI: 10.2210/pdb5x7h/pdb
• Related: 5X7G 5X7O 5X7P 5X7Q 5X7R 5X7S
• Descriptor: Cycloisomaltooligosaccharide glucanotransferase, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: glydoside hydrolase family 66, carbohydrate-binding module family 35, transferase
• Biological source: Paenibacillus sp. 598K
• Total number of polymer chains: 1
• Total formula weight: 83634.14

Authors

Fujimoto, Z.,Kishine, N.,Suzuki, N.,Suzuki, R.,Momma, M.,Funane, K. (deposition date: 2017-02-26, release date: 2017-04-26, Last modification date: 2023-11-22)

Primary citation

Fujimoto, Z.,Kishine, N.,Suzuki, N.,Suzuki, R.,Mizushima, D.,Momma, M.,Kimura, K.,Funane, K.
Isomaltooligosaccharide-binding structure ofPaenibacillussp. 598K cycloisomaltooligosaccharide glucanotransferase
Biosci. Rep., 37:-, 2017

PubMed Abstract: sp. 598K cycloisomaltooligosaccharide glucanotransferase (CITase), a member of glycoside hydrolase family 66 (GH66), catalyses the intramolecular transglucosylation of dextran to produce CIs with seven or more degrees of polymerization. To clarify the cyclization reaction and product specificity of the enzyme, we determined the crystal structure of PsCITase. The core structure of PsCITase consists of four structural domains: a catalytic (β/α)-domain and three β-domains. A family 35 carbohydrate-binding module (first CBM35 region of sp. 598K CITase, (PsCBM35-1)) is inserted into and protrudes from the catalytic domain. The ligand complex structure of PsCITase prepared by soaking the crystal with cycloisomaltoheptaose yielded bound sugars at three sites: in the catalytic cleft, at the joint of the PsCBM35-1 domain and at the loop region of PsCBM35-1. In the catalytic site, soaked cycloisomaltoheptaose was observed as a linear isomaltoheptaose, presumably a hydrolysed product from cycloisomaltoheptaose by the enzyme and occupied subsites -7 to -1. Beyond subsite -7, three glucose moieties of another isomaltooiligosaccharide were observed, and these positions are considered to be distal subsites -13 to -11. The third binding site is the canonical sugar-binding site at the loop region of PsCBM35-1, where the soaked cycloisomaltoheptaose is bound. The structure indicated that the concave surface between the catalytic domain and PsCBM35-1 plays a guiding route for the long-chained substrate at the cyclization reaction.

PubMed: 28385816
DOI: 10.1042/BSR20170253

Experimental method

X-RAY DIFFRACTION (2.6 Å)