5WWL

Crystal structure of the Schizogenesis pombe kinetochore Mis12C subcomplex

Summary for 5WWL

• Entry DOI: 10.2210/pdb5wwl/pdb
• Descriptor: Centromere protein mis12, Kinetochore protein nnf1 (2 entities in total)
• Functional Keywords: kinetochore mis12c, cell cycle
• Biological source: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); More
• Cellular location: Chromosome, centromere : Q9Y738; Nucleus : Q09858
• Total number of polymer chains: 2
• Total formula weight: 45543.05

Authors

Wang, C.,Zhou, X.,Wu, M.,Zhang, X.,Zang, J. (deposition date: 2017-01-02, release date: 2017-11-15, Last modification date: 2024-03-20)

Primary citation

Zhou, X.,Zheng, F.,Wang, C.,Wu, M.,Zhang, X.,Wang, Q.,Yao, X.,Fu, C.,Zhang, X.,Zang, J.
Phosphorylation of CENP-C by Aurora B facilitates kinetochore attachment error correction in mitosis.
Proc. Natl. Acad. Sci. U.S.A., 114:E10667-E10676, 2017

PubMed Abstract: Kinetochores are superprotein complexes that orchestrate chromosome segregation via a dynamic interaction with spindle microtubules. A physical connection between CENP-C and the Mis12-Ndc80-Knl1 (KMN) protein network is an important pathway that is used to assemble kinetochores on CENP-A nucleosomes. Multiple outer kinetochore components are phosphorylated by Aurora B kinase to activate the spindle assembly checkpoint (SAC) and to ensure accurate chromosome segregation. However, it is unknown whether Aurora B can phosphorylate inner kinetochore components to facilitate proper mitotic chromosome segregation. Here, we reported the structure of the fission yeast Mis12-Nnf1 complex and showed that N-terminal residues 26-50 in Cnp3 (the CENP-C homolog of ) are responsible for interacting with the Mis12 complex. Interestingly, Thr28 of Cnp3 is a substrate of Ark1 (the Aurora B homolog of ), and phosphorylation impairs the interaction between the Cnp3 and Mis12 complex. The expression of a phosphorylation-mimicking Cnp3 mutant results in defective chromosome segregation due to improper kinetochore assembly. These results establish a previously uncharacterized regulatory mechanism involved in CENP-C-Mis12-facilitated kinetochore attachment error correction to ensure accurate chromosome segregation during mitosis.

PubMed: 29180432
DOI: 10.1073/pnas.1710506114

Experimental method

X-RAY DIFFRACTION (2.4 Å)