Summary for 6CQ8
| Entry DOI | 10.2210/pdb6cq8/pdb |
| Related | 6CQ6 |
| Descriptor | Potassium channel subfamily K member 2, POTASSIUM ION, HEXADECANE, ... (6 entities in total) |
| Functional Keywords | trek-1 ion channel k2p, transport protein |
| Biological source | Mus musculus (Mouse) |
| Total number of polymer chains | 2 |
| Total formula weight | 72728.52 |
| Authors | Lolicato, M.,Minor, D.L. (deposition date: 2018-03-14, release date: 2018-03-28, Last modification date: 2024-10-23) |
| Primary citation | Lolicato, M.,Arrigoni, C.,Mori, T.,Sekioka, Y.,Bryant, C.,Clark, K.A.,Minor, D.L. K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature, 547:364-368, 2017 Cited by PubMed Abstract: Polymodal thermo- and mechanosensitive two-pore domain potassium (K) channels of the TREK subfamily generate 'leak' currents that regulate neuronal excitability, respond to lipids, temperature and mechanical stretch, and influence pain, temperature perception and anaesthetic responses. These dimeric voltage-gated ion channel (VGIC) superfamily members have a unique topology comprising two pore-forming regions per subunit. In contrast to other potassium channels, K channels use a selectivity filter 'C-type' gate as the principal gating site. Despite recent advances, poor pharmacological profiles of K channels limit mechanistic and biological studies. Here we describe a class of small-molecule TREK activators that directly stimulate the C-type gate by acting as molecular wedges that restrict interdomain interface movement behind the selectivity filter. Structures of K2.1 (also known as TREK-1) alone and with two selective K2.1 (TREK-1) and K10.1 (TREK-2) activators-an N-aryl-sulfonamide, ML335, and a thiophene-carboxamide, ML402-define a cryptic binding pocket unlike other ion channel small-molecule binding sites and, together with functional studies, identify a cation-π interaction that controls selectivity. Together, our data reveal a druggable K site that stabilizes the C-type gate 'leak mode' and provide direct evidence for K selectivity filter gating. PubMed: 28693035DOI: 10.1038/nature22988 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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