5O2X

Extended catalytic domain of H. jecorina LPMO9A a.k.a EG4

Summary for 5O2X

• Entry DOI: 10.2210/pdb5o2x/pdb
• Descriptor: Glycoside hydrolase family 61, 2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose, ... (6 entities in total)
• Functional Keywords: lytic polysaccharide monooxygenase trichoderma reesei eg4, oxidoreductase
• Biological source: Trichoderma reesei QM6a
• Total number of polymer chains: 1
• Total formula weight: 29629.46

Authors

Hansson, H.,Karkehabadi, S.,Mikkelsen, N.E.,Sandgren, M.,Kelemen, B.,Kaper, T. (deposition date: 2017-05-23, release date: 2017-09-20, Last modification date: 2020-07-29)

Primary citation

Hansson, H.,Karkehabadi, S.,Mikkelsen, N.,Douglas, N.R.,Kim, S.,Lam, A.,Kaper, T.,Kelemen, B.,Meier, K.K.,Jones, S.M.,Solomon, E.I.,Sandgren, M.
High-resolution structure of a lytic polysaccharide monooxygenase from Hypocrea jecorina reveals a predicted linker as an integral part of the catalytic domain.
J. Biol. Chem., 292:19099-19109, 2017

PubMed Abstract: For decades, the enzymes of the fungus have served as a model system for the breakdown of cellulose. Three-dimensional structures for almost all cellulose-degrading enzymes are available, except for LPMO9A, belonging to the AA9 family of lytic polysaccharide monooxygenases (LPMOs). These enzymes enhance the hydrolytic activity of cellulases and are essential for cost-efficient conversion of lignocellulosic biomass. Here, using structural and spectroscopic analyses, we found that native LPMO9A contains a catalytic domain and a family-1 carbohydrate-binding module (CBM1) connected via a linker sequence. A C terminally truncated variant of LPMO9A containing 21 residues of the predicted linker was expressed at levels sufficient for analysis. Here, using structural, spectroscopic, and biochemical analyses, we found that this truncated variant exhibited reduced binding to and activity on cellulose compared with the full-length enzyme. Importantly, a 0.95-Å resolution X-ray structure of truncated LPMO9A revealed that the linker forms an integral part of the catalytic domain structure, covering a hydrophobic patch on the catalytic AA9 module. We noted that the oxidized catalytic center contains a Cu(II) coordinated by two His ligands, one of which has a His-brace in which the His-1 terminal amine group also coordinates to a copper. The final equatorial position of the Cu(II) is occupied by a water-derived ligand. The spectroscopic characteristics of the truncated variant were not measurably different from those of full-length LPMO9A, indicating that the presence of the CBM1 module increases the affinity of LPMO9A for cellulose binding, but does not affect the active site.

PubMed: 28900033
DOI: 10.1074/jbc.M117.799767

Experimental method

X-RAY DIFFRACTION (0.95 Å)