5NJQ
Mix-and-diffuse serial synchrotron crystallography: structure of N,N',N''-Triacetylchitotriose bound to Lysozyme with 1s time-delay, phased with 4ET8
Summary for 5NJQ
| Entry DOI | 10.2210/pdb5njq/pdb |
| Related PRD ID | PRD_900017 |
| Descriptor | Lysozyme C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (5 entities in total) |
| Functional Keywords | competitive inhibitor glycoside hydrolase, hydrolase |
| Biological source | Gallus gallus (Chicken) |
| Cellular location | Secreted: P00698 |
| Total number of polymer chains | 1 |
| Total formula weight | 15052.65 |
| Authors | Oberthuer, D.,Meents, A.,Beyerlein, K.R.,Chapman, H.N.,Lieseke, J. (deposition date: 2017-03-29, release date: 2017-10-18, Last modification date: 2024-11-13) |
| Primary citation | Beyerlein, K.R.,Dierksmeyer, D.,Mariani, V.,Kuhn, M.,Sarrou, I.,Ottaviano, A.,Awel, S.,Knoska, J.,Fuglerud, S.,Jonsson, O.,Stern, S.,Wiedorn, M.O.,Yefanov, O.,Adriano, L.,Bean, R.,Burkhardt, A.,Fischer, P.,Heymann, M.,Horke, D.A.,Jungnickel, K.E.J.,Kovaleva, E.,Lorbeer, O.,Metz, M.,Meyer, J.,Morgan, A.,Pande, K.,Panneerselvam, S.,Seuring, C.,Tolstikova, A.,Lieske, J.,Aplin, S.,Roessle, M.,White, T.A.,Chapman, H.N.,Meents, A.,Oberthuer, D. Mix-and-diffuse serial synchrotron crystallography. IUCrJ, 4:769-777, 2017 Cited by PubMed Abstract: Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources. PubMed: 29123679DOI: 10.1107/S2052252517013124 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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