5LSH

human lysozyme in complex with a tetrasaccharide fragment of the O-chain of LPS from Klebsiella pneumoniae

Summary for 5LSH

• Entry DOI: 10.2210/pdb5lsh/pdb
• Descriptor: Lysozyme C, alpha-D-galactopyranose-(1-3)-beta-D-galactofuranose-(1-3)-alpha-D-galactopyranose-(1-3)-propyl beta-D-galactofuranoside, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: lectin, complex, lps, o-chain, sugar binding protein
• Biological source: Homo sapiens (Human)
• Cellular location: Secreted: P61626
• Total number of polymer chains: 1
• Total formula weight: 15558.70

Authors

Zhang, R.,Nifantiev, N.E.,Krylov, V.,Luetteke, T.,Scheidig, A.J.,Siebert, H.-C. (deposition date: 2016-08-26, release date: 2017-06-21, Last modification date: 2024-01-17)

Primary citation

Zhang, R.,Wu, L.,Eckert, T.,Burg-Roderfeld, M.,Rojas-Macias, M.A.,Lutteke, T.,Krylov, V.B.,Argunov, D.A.,Datta, A.,Markart, P.,Guenther, A.,Norden, B.,Schauer, R.,Bhunia, A.,Enani, M.A.,Billeter, M.,Scheidig, A.J.,Nifantiev, N.E.,Siebert, H.C.
Lysozyme's lectin-like characteristics facilitates its immune defense function.
Q. Rev. Biophys., 50:e9-e9, 2017

PubMed Abstract: Interactions between human lysozyme (HL) and the lipopolysaccharide (LPS) of Klebsiella pneumoniae O1, a causative agent of lung infection, were identified by surface plasmon resonance. To characterize the molecular mechanism of this interaction, HL binding to synthetic disaccharides and tetrasaccharides representing one and two repeating units, respectively, of the O-chain of this LPS were studied. pH-dependent structural rearrangements of HL after interaction with the disaccharide were observed through nuclear magnetic resonance. The crystal structure of the HL-tetrasaccharide complex revealed carbohydrate chain packing into the A, B, C, and D binding sites of HL, which primarily occurred through residue-specific, direct or water-mediated hydrogen bonds and hydrophobic contacts. Overall, these results support a crucial role of the Glu35/Asp53/Trp63/Asp102 residues in HL binding to the tetrasaccharide. These observations suggest an unknown glycan-guided mechanism that underlies recognition of the bacterial cell wall by lysozyme and may complement the HL immune defense function.

PubMed: 29233221
DOI: 10.1017/S0033583517000075

Experimental method

X-RAY DIFFRACTION (1.061 Å)