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5L1P

X-ray Structure of Cytochrome P450 PntM with Pentalenolactone

Summary for 5L1P
Entry DOI10.2210/pdb5l1p/pdb
Related5L1O 5L1Q 5L1R 5L1S 5L1T 5L1U 5L1V 5L1W
DescriptorPentalenolactone synthase, PROTOPORPHYRIN IX CONTAINING FE, pentalenolactone, ... (4 entities in total)
Functional Keywordspntm, cytochrome p450, pentalenolactone, oxidoreductase
Biological sourceStreptomyces arenae
Total number of polymer chains1
Total formula weight45397.58
Authors
Duan, L.,Jogl, G.,Cane, D.E. (deposition date: 2016-07-29, release date: 2016-09-14, Last modification date: 2023-10-04)
Primary citationDuan, L.,Jogl, G.,Cane, D.E.
The Cytochrome P450-Catalyzed Oxidative Rearrangement in the Final Step of Pentalenolactone Biosynthesis: Substrate Structure Determines Mechanism.
J.Am.Chem.Soc., 138:12678-12689, 2016
Cited by
PubMed Abstract: The final step in the biosynthesis of the sesquiterpenoid antibiotic pentalenolactone (1) is the highly unusual cytochrome P450-catalyzed, oxidative rearrangement of pentalenolactone F (2), involving the transient generation and rearrangement of a neopentyl cation. In Streptomyces arenae this reaction is catalyzed by CYP161C2 (PntM), with highly conserved orthologs being present in at least 10 other Actinomycetes. Crystal structures of substrate-free PntM, as well as PntM with bound substrate 2, product 1, and substrate analogue 6,7-dihydropentalenolactone F (7) revealed interactions of bound ligand with three residues, F232, M77, and M81 that are unique to PntM and its orthologs and absent from essentially all other P450s. Site-directed mutagenesis, ligand-binding measurements, steady-state kinetics, and reaction product profiles established there is no special stabilization of reactive cationic intermediates by these side chains. Reduced substrate analogue 7 did not undergo either oxidative rearrangement or simple hydroxylation, suggesting that the C1 carbocation is not anchimerically stabilized by the 6,7-double bond of 2. The crystal structures also revealed plausible proton relay networks likely involved in the generation of the key characteristic P450 oxidizing species, Compound I, and in mediating stereospecific deprotonation of H-3re of the substrate. We conclude that the unusual carbocation intermediate results from outer shell electron transfer from the transiently generated C1 radical to the tightly paired heme-•Fe(3+)-OH radical species. The oxidative electron transfer is kinetically dominant as a result of the unusually strong steric barrier to oxygen rebound to the neopentyl center C-1si, which is flanked on each neighboring carbon by syn-axial substituents.
PubMed: 27588339
DOI: 10.1021/jacs.6b08610
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.28 Å)
Structure validation

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