5JNQ

MraY tunicamycin complex

Summary for 5JNQ

• Entry DOI: 10.2210/pdb5jnq/pdb
• Descriptor: Phospho-N-acetylmuramoyl-pentapeptide-transferase, Tunicamycin, PALMITIC ACID, ... (5 entities in total)
• Functional Keywords: membrane protein, antibiotic, pnpt, transferase-antibiotic complex, transferase/antibiotic
• Biological source: Clostridium bolteae 90A9
• Total number of polymer chains: 1
• Total formula weight: 42569.24

Authors

Johansson, P. (deposition date: 2016-04-30, release date: 2017-01-11, Last modification date: 2024-01-10)

Primary citation

Hakulinen, J.K.,Hering, J.,Branden, G.,Chen, H.,Snijder, A.,Ek, M.,Johansson, P.
MraY-antibiotic complex reveals details of tunicamycin mode of action.
Nat. Chem. Biol., 13:265-267, 2017

PubMed Abstract: The rapid increase of antibiotic resistance has created an urgent need to develop novel antimicrobial agents. Here we describe the crystal structure of the promising bacterial target phospho-N-acetylmuramoyl-pentapeptide translocase (MraY) in complex with the nucleoside antibiotic tunicamycin. The structure not only reveals the mode of action of several related natural-product antibiotics but also gives an indication on the binding mode of the MraY UDP-MurNAc-pentapeptide and undecaprenyl-phosphate substrates.

PubMed: 28068312
DOI: 10.1038/nchembio.2270

Experimental method

X-RAY DIFFRACTION (2.6 Å)