5H47
Crystal structure of AOL complexed with 2-MeSe-Fuc
Summary for 5H47
| Entry DOI | 10.2210/pdb5h47/pdb |
| Related | 5EO7 5EO8 |
| Descriptor | Uncharacterized protein, methyl 6-deoxy-2-Se-methyl-2-seleno-alpha-L-galactopyranoside (3 entities in total) |
| Functional Keywords | seleno-fucoses, phaing, lectin, aspergillus oryzae, sugar binding protein |
| Biological source | Aspergillus oryzae RIB40 (Yellow koji mold) |
| Total number of polymer chains | 12 |
| Total formula weight | 433279.84 |
| Authors | Kato, R.,Nishikawa, Y.,Makyio, H. (deposition date: 2016-10-31, release date: 2017-01-25, Last modification date: 2024-03-20) |
| Primary citation | Shimabukuro, J.,Makyio, H.,Suzuki, T.,Nishikawa, Y.,Kawasaki, M.,Imamura, A.,Ishida, H.,Ando, H.,Kato, R.,Kiso, M. Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing Bioorg. Med. Chem., 25:1132-1142, 2017 Cited by PubMed Abstract: Selenium-incorporated fucoses (seleno-fucoses) differing in the position of the seleno-substituent were synthesized and applied to the X-ray structural determination of a carbohydrate-lectin complex using single/multi-wavelength anomalous dispersion (SAD/MAD) phasing. The hydroxyl groups at the C-1, -2, -3 and -4 position of fucose were individually substituted with a methylseleno group via a transacetalization reaction using MeSeCHOBn or by an S2 reaction with TolSe equivalents to afford the corresponding MeSe-fucose. The three-dimensional structures of a fucose-binding lectin complexed with several of these MeSe-fucoses have been determined by SAD/MAD phasing by utilizing the diffraction of selenium in the bound MeSe-fucoses. PubMed: 28041800DOI: 10.1016/j.bmc.2016.12.021 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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