4ZZP

Dictyostelium purpureum cellobiohydrolase Cel7A apo structure

4ZZP の概要

• エントリーDOI: 10.2210/pdb4zzp/pdb
• 関連するPDBエントリー: 4ZZQ
• 分子名称: CELLULOSE 1,4-BETA-CELLOBIOSIDASE, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase, cellobiohydrolase, cellulase
• 由来する生物種: DICTYOSTELIUM PURPUREUM (CELLULAR SLIME MOLD)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95631.47

構造登録者

Momeni, M.H.,Hobdey, S.E.,Knott, B.,Beckham, G.T.,Stahlberg, J. (登録日: 2015-04-13, 公開日: 2016-04-13, 最終更新日: 2024-11-13)

主引用文献

Hobdey, S.E.,Knott, B.C.,Haddad Momeni, M.,Taylor, L.E.,Borisova, A.S.,Podkaminer, K.K.,Vanderwall, T.A.,Himmel, M.E.,Decker, S.R.,Beckham, G.T.,Stahlberg, J.
Biochemical and Structural Characterization of Two Dictyostelium Cellobiohydrolases from the Amoebozoa Kingdom Reveal a High Conservation between Distant Phylogenetic Trees of Life.
Appl.Environ.Microbiol., 82:3395-, 2016

PubMed Abstract: Glycoside hydrolase family 7 (GH7) cellobiohydrolases (CBHs) are enzymes commonly employed in plant cell wall degradation across eukaryotic kingdoms of life, as they provide significant hydrolytic potential in cellulose turnover. To date, many fungal GH7 CBHs have been examined, yet many questions regarding structure-activity relationships in these important natural and commercial enzymes remain. Here, we present the crystal structures and a biochemical analysis of two GH7 CBHs from social amoeba: Dictyostelium discoideum Cel7A (DdiCel7A) and Dictyostelium purpureum Cel7A (DpuCel7A). DdiCel7A and DpuCel7A natively consist of a catalytic domain and do not exhibit a carbohydrate-binding module (CBM). The structures of DdiCel7A and DpuCel7A, resolved to 2.1 Å and 2.7 Å, respectively, are homologous to those of other GH7 CBHs with an enclosed active-site tunnel. Two primary differences between the Dictyostelium CBHs and the archetypal model GH7 CBH, Trichoderma reesei Cel7A (TreCel7A), occur near the hydrolytic active site and the product-binding sites. To compare the activities of these enzymes with the activity of TreCel7A, the family 1 TreCel7A CBM and linker were added to the C terminus of each of the Dictyostelium enzymes, creating DdiCel7ACBM and DpuCel7ACBM, which were recombinantly expressed in T. reesei DdiCel7ACBM and DpuCel7ACBM hydrolyzed Avicel, pretreated corn stover, and phosphoric acid-swollen cellulose as efficiently as TreCel7A when hydrolysis was compared at their temperature optima. The Ki of cellobiose was significantly higher for DdiCel7ACBM and DpuCel7ACBM than for TreCel7A: 205, 130, and 29 μM, respectively. Taken together, the present study highlights the remarkable degree of conservation of the activity of these key natural and industrial enzymes across quite distant phylogenetic trees of life.

PubMed: 27037126
DOI: 10.1128/AEM.00163-16

実験手法

X-RAY DIFFRACTION (2.7 Å)