4ZV7

Crystal structure of hexagonal form of lipase B from Candida antarctica

Summary for 4ZV7

• Entry DOI: 10.2210/pdb4zv7/pdb
• Related: 1TCA 1TCB 1TCC 3W9B
• Descriptor: Lipase B, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: cal-b, hexagonal form, hydrolase
• Biological source: Candida antarctica (Yeast)
• Total number of polymer chains: 1
• Total formula weight: 33464.64

Authors

Strzelczyk, P.,Blaszczyk, J.,Bujacz, G. (deposition date: 2015-05-18, release date: 2016-01-13, Last modification date: 2024-11-13)

Primary citation

Strzelczyk, P.,Bujacz, G.D.,Kiebasinski, P.,Baszczyk, J.
Crystal and molecular structure of hexagonal form of lipase B from Candida antarctica.
Acta Biochim.Pol., 63:103-109, 2016

PubMed Abstract: During crystallization screenings of commercially available hydrolytic enzymes, the new, hexagonal crystal form of CAL-B, has been discovered and hereby reported. The NAG molecules, which were closing the glycosylation site in the orthorhombic form, in hexagonal structure make the glycosylation site open. It is unknown whether the opening and closing of the glycosylation site by the 'lid' NAG molecules, could be related to the opening and closing of the active center of the enzyme upon substrate binding and product release.

PubMed: 26716135
DOI: 10.18388/abp.2015_1065

Experimental method

X-RAY DIFFRACTION (2 Å)