4ZV7

Crystal structure of hexagonal form of lipase B from Candida antarctica

4ZV7 の概要

• エントリーDOI: 10.2210/pdb4zv7/pdb
• 関連するPDBエントリー: 1TCA 1TCB 1TCC 3W9B
• 分子名称: Lipase B, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: cal-b, hexagonal form, hydrolase
• 由来する生物種: Candida antarctica (Yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33464.64

構造登録者

Strzelczyk, P.,Blaszczyk, J.,Bujacz, G. (登録日: 2015-05-18, 公開日: 2016-01-13, 最終更新日: 2024-11-13)

主引用文献

Strzelczyk, P.,Bujacz, G.D.,Kiebasinski, P.,Baszczyk, J.
Crystal and molecular structure of hexagonal form of lipase B from Candida antarctica.
Acta Biochim.Pol., 63:103-109, 2016

PubMed Abstract: During crystallization screenings of commercially available hydrolytic enzymes, the new, hexagonal crystal form of CAL-B, has been discovered and hereby reported. The NAG molecules, which were closing the glycosylation site in the orthorhombic form, in hexagonal structure make the glycosylation site open. It is unknown whether the opening and closing of the glycosylation site by the 'lid' NAG molecules, could be related to the opening and closing of the active center of the enzyme upon substrate binding and product release.

PubMed: 26716135
DOI: 10.18388/abp.2015_1065

実験手法

X-RAY DIFFRACTION (2 Å)