4ZUH

Complex structure of PEDV 3CLpro mutant (C144A) with a peptide substrate.

4ZUH の概要

• エントリーDOI: 10.2210/pdb4zuh/pdb
• 分子名称: PEDV 3C-Like protease, peptide substrate SAVLQSGF (3 entities in total)
• 機能のキーワード: 3c-like protease, hydrolase-peptide complex, hydrolase/peptide
• 由来する生物種: Porcine epidemic diarrhea virus; 詳細
• 細胞内の位置: Host cytoplasm, host perinuclear region . Host membrane ; Multi-pass membrane protein : K4L9I6
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 67722.53

構造登録者

Ye, G.,Fu, Z.F.,Peng, G.Q. (登録日: 2015-05-16, 公開日: 2016-06-15, 最終更新日: 2024-03-20)

主引用文献

Ye, G.,Deng, F.,Shen, Z.,Luo, R.,Zhao, L.,Xiao, S.,Fu, Z.F.,Peng, G.
Structural basis for the dimerization and substrate recognition specificity of porcine epidemic diarrhea virus 3C-like protease.
Virology, 494:225-235, 2016

PubMed Abstract: Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here, we present the crystal structures of PEDV 3CL(pro) and a molecular complex between an inactive PEDV 3CL(pro) variant C144A bound to a peptide substrate. Structural characterization, mutagenesis and biochemical analysis reveal the substrate-binding pockets and the residues that comprise the active site of PEDV 3CL(pro). The dimerization of PEDV 3CL(pro) is similar to that of other Alphacoronavirus 3CL(pro)s but has several differences from that of SARS-CoV 3CL(pro) from the genus Betacoronavirus. Furthermore, the non-conserved motifs in the pockets cause different cleavage of substrate between PEDV and SARS-CoV 3CL(pro)s, which may provide new insights into the recognition of substrates by 3CL(pro)s in various coronavirus genera.

PubMed: 27128350
DOI: 10.1016/j.virol.2016.04.018

実験手法

X-RAY DIFFRACTION (2.394 Å)